Rapid adipose tissue expansion induces proliferation of memory adipose tissue T cells

Abstract Obesity is recognized as a pro-inflammatory state featuring immune dysregulation and metabolic disease. Obesity-induced inflammation observed in the adipose tissue of humans mice. Adipose T cells (ATTs) are dynamically shifted from Th2 lean to an influx CD8 +and Th1 that promote obese state. Knowing this, many gaps remain our understanding ATT generation, maintenance, expansion, regulation tissue. Specifically, it unknown if naïve recruited stimulated within or resident memory ATTs, which abundant state, during initiation We hypothesized ATTs respond early fat expansion elicited upon obesity induction. To test we used short-term DIO male mouse model assessed proliferation high-fat diet (HFD) feeding. Mice on HFD gained significantly more total body, epidydimal white (eWAT), inguinal WAT (iWAT) weight compared normal no increase frequency with HFD. Conversely, significant 1 week eWAT, iWAT omental WAT. This was restored levels by 2 weeks These data suggest uniquely intensely signals present HFD-induced rapid expansion. Ongoing studies seek determine there sex differences examining dynamics female Further, signal(s) drive identify targets suppress obesity-associated inflammation. Supported grants NIH (K12 GM111725, R01 DK090262).